Stable Haploid Cultured Cell Lines from frog Embryos

Stable haploid cultured cell lines from frog embryos.

Two haploid cell lines have been established from androgenetic embryos of the frog, Rana pipiens; one line has been maintained in culture for 150 generations, the other for 200 generations. Karyotypes of the two lines agree well with the standard for the species although some chromosomes show small differences in length. The cells multiply in the same defined basal medium used for culture of ot...

Establishment of pluripotential cell lines from haploid mouse embryos.

Eggs from 129 SvE and (C57BL x CBA)F1 hybrid female mice were activated parthenogenetically following their exposure to a 7% solution of ethanol in PBS. Only the haploid class which developed a single pronucleus following second polar body extrusion was examined further. These eggs were transferred to suitable recipients and 'delayed' blastocysts subsequently recovered. The 'delayed' blastocyst...

Characterization of Nucleoprotein Extracted from Human Influenza A Virus Cultured in Two Different Cell Lines

Background and Aims: Influenza virus nucleoprotein (NP) has the capacity to be used as subunit vaccine, but little is known about the impact of different cultures on its structure. In the present study we aimed to evaluate and compare the Isoelectric focusing (IEF) property of extracted viral nucleoproteins derived from Madin Darby canine kidney (MDCK) cell line and embryonated chicken eggs (EC...

Cell Number in Haploid, Diploid and Polyploid Mouse Embryos

P-118: Developmental Competence of Ovine Embryos Derived from Frozen Oocytes Co-Cultured with Embryos Derived from Fresh Oocytes

Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...